This technology uses a real-time polymerase chain reaction (reverse transcriptase -quantitative polymerase chain reaction or RT-qPCR ) for the detection of human parechovirus (HPeV) in blood and cerebrospinal fluid (CSF) samples. The purpose of this technique is to quantify the viral particles by determining the number of HPeV genome copies present in the patient's blood or CSF. HPeVs are often the cause of pediatric infections in immunocompetent, as well immunocompromized, individuals.
Targeted patient groups for this test include:
- patients under 18 years of age with clinical manifestations of meningitis or encephalitis, with or without compatible imaging findings
- patients under three months with a clinical presentation of sepsis, with or without fulminant hepatitis or coagulopathy
- adults with a clinical presentation of encephalitis; the test may then be specifically requested (otherwise, adults will be excluded).
Information on this lab test was originally published in French by the Institut national d’excellence en santé et en services sociaux (INESSS). CADTH is pleased to provide this translated version. To view the original report in French, click here. For more information on the assessment process, visit www.inesss.qc.ca/en/activites/procedures-de-biologie-medicale/assessment-mechanism-for-medical-biology-tests-at-inesss.html.